Part-time Staff Research Associate
UC San Francisco (UCSF) · San Francisco, California, United States
Our lab aims to understand the molecular mechanisms of lung development and repair. The individual hired will primarily be involved in phenotypic and molecular analysis of mouse models of lung development and repair. This work requires tissue collection, processing, and analysis, molecular biology and biochemistry techniques, and data analysis, including transcriptomics and epigenomics.
%
of time
Essential Function (Yes/No)
Key Responsibilities
(To be completed by Supervisor)
25
YesUnder supervision, perform molecular biology/biochemistry experiments
• genomic DNA isolation
• RNA isolation
• protein isolation
• agarose gel electrophoresis
• isolation of DNA from agarose gel
• ligation of DNA
• transformation of DNA
• mini- and maxi-preparation of DNA
• PCR and qPCR
• Western blotting
• sgRNA design
25
YesUnder supervision, perform phenotypic and molecular analysis of mouse models of lung development and repair, tissue collection, processing and analysis
• general mouse husbandry
• lung dissection (including perfusion)
• fixation, embedding, and sectioning of lungs
• antibody staining of lung tissues (whole mount and
section)
• EdU and Tunnel staining of lung tissues
25
YesUnder supervision, perform cell culture and cell-based studies
• lung cell isolation
• organoid culture
• transduction of lung cells with candidate genes or libraries
• CRISPR and CRISPR screens of lung cells
25
YesUnder supervision, perform data analysis
• Bulk RNA-seq
• scRNA-seq
• Multi-omics
• ATAC-seq
• Spatial transcriptomics
• CUT&RUN
• CRISPR screens
100%
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Required Qualifications
- BA/BS in a related science and knowledge of, or experience with, the basic techniques or methods required by the position; and/or an equivalent combination of education and experience.
- Attention to detail
- Ability to follow instructions and protocols
- Ability to keep track of animal numbers during all experimental manipulations
- Time management skills to complete the assigned tasks within 16 hours
- Excellent written and verbal communication skills
- Excellent interpersonal skills
- Excellent organizational skills to aid in the smooth operation of the laboratory by maintaining adequate supplies and equipment operation
- Ability to handle unpleasant elements and wear protective clothing or equipment
Preferred Qualifications
- Knowledge and experience in molecular biology and biochemistry
- Knowledge and experience in cell-based studies
- Knowledge and experience in animal studies
- Knowledge and experience in data analysis